So I have a question. Rather than take all the time to sanitize every square inch of your pad with rubbing alcohol to inoculate or I'm sorry to inject a spore bag. Because there's no oxygen in water c... View MoreSo I have a question. Rather than take all the time to sanitize every square inch of your pad with rubbing alcohol to inoculate or I'm sorry to inject a spore bag. Because there's no oxygen in water couldn't you submerge your spore bag and your syringe and therefore eliminate all chances of contamination that way or would it have to be hot boiling water?
Water on a molecular level is H²O. The O stands for oxygen so I don't think it is correct to say there is no oxygen in water. Your best bet is to make a SAB(Still Air Box). But if you buy self sealing injection ports and just sterilize the syringe before injection im sure you could just do it out in... View MoreWater on a molecular level is H²O. The O stands for oxygen so I don't think it is correct to say there is no oxygen in water. Your best bet is to make a SAB(Still Air Box). But if you buy self sealing injection ports and just sterilize the syringe before injection im sure you could just do it out in the open because the bag can stayed sealed in that case.
there are aerobic and anaerobic bacteria, the latter won't die under water as they do not need oxygen. so there is a technique called cold pasteurisation in which you submerge your substrate, straw for example, for 24-36 hours.
then all the aerobic bacteria die off, and as you take it out of the wat... View Morethere are aerobic and anaerobic bacteria, the latter won't die under water as they do not need oxygen. so there is a technique called cold pasteurisation in which you submerge your substrate, straw for example, for 24-36 hours.
then all the aerobic bacteria die off, and as you take it out of the water, the anaerobic bac. come into contact with oxygen, which they don't like, and then die off.
but this is not a sterilisation process but merely a pasteurisation.