Question: Firstly, I havent a CLUE what I'm doing with agar or the different types or why to chose this one or that. The situation is this. I have a bunch of prints i made last year. I failed my first... View MoreQuestion: Firstly, I havent a CLUE what I'm doing with agar or the different types or why to chose this one or that. The situation is this. I have a bunch of prints i made last year. I failed my first wbs tek using these prints, (cobweb mold). So i ordered some potato agar dishes, just came in the mail. I dont know whether to make another syringe to hydrate the spores then put to agar or straight spores to agar. Should i use as few spores as possible on the agar or a lot? Should i use multiple prints on the same dish or one print per dish or the same print on multiple dishes and how many places on the dish should i put spores? I am anticipating having to transfer to another dish once it gets going to make sure its clean but how many times should i transfer before i can trust i have a clean culture? I see so few posts on closeup images of mycelium grown out on agar and how to know the best portion to cut and transfer. I hear "sectoring" but havent seen examples. Is there a book or something with images on this? Sorry for all the questions. Im new to the hobby and i have read so many conflicting opinions. I want to get this right but also understand why i am taking the steps suggested. My common sense is much better if i understand the process. I really appreciate those of you that offer help and explanation. Everyone here is winning. Im new here but i havent seen a single rude comment. I love yall!
Thats corn on the cob correct? This is my first attempt. Wild to print to syringe pcd sterilized inside and out still air box rtv and 5/16 tyvek poot hole. I figure the dirty prints were probably just... View MoreThats corn on the cob correct? This is my first attempt. Wild to print to syringe pcd sterilized inside and out still air box rtv and 5/16 tyvek poot hole. I figure the dirty prints were probably just that, dirty. Ordered agar dishes. Any good "heres how you cleanup dirty spores using agar" teks out there?
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