I found my main issue in mushy grow progress. Since I use agar plates bought online and not DIY, I can actually create some cultures. I thought my mistake was working not sterile enough but in the end I am just unable to make some sterile agar plates . I just have to admit I cannot do every single step by myself. What I still need to learn is, how to interpret culture growth and to see if it's contaminated or clean. Also I would like to know more about different kind of growth. Does anybody has some available information for that? Or maybe video recommendations?
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DBL-D
DO- flame sterilize the scalpel. I use oven flow for pouring agar plates and it works out great. Do you turn the agar plates upside down after they solidify? The condensation from the sides of the cup sometimes contaminates the agar.
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June 5, 2023
AcidFriendly
Thank you GaiaGazing for your answer! PE6 and GT I transfered from a clean plate. I don't understand why they grow like this. M&T actually came from a contaminated plate. DIY flow hood will be my next step, also I do flame sterilize my blade. Actually what DBL-D mentioned could be my problem with ag... View More
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June 6, 2023