Hoping to pick your brains—I am using a LME agar mix (15g of LME, 10g of agar, 500 ML of hot water) and keeping these cups at room temp which is roughly 71 degrees. They have been growing very slowly. Are there any tips to speed up the process such as keeping them at a different temperature or using ingredients that are more nutritious? Thanks
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Batman419
You want to aim for around 4%-5% nutrients. Maybe throw a couple teaspoons of brewed coffee in the water. Higher temp =faster growth for good and bad bacteria, so be careful over 78. I did 82 last year and fought trich like you wouldnt believe. Also, if APE is anything like PE, its just a slow strai... View More
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February 23, 2022
Donniebrasco1
You can do oatmeal recipe i think i got from mr madness 500 ml water boil 35gs oatmeal let sit strain water and fill to 500 ml some of the water will be absorbed by the oats add 12 g agar 2 g nutritional yeast powder 20 mins 15 psi
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February 23, 2022
Batman419
I use that oatmeal recipe too. Minus the yeast
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February 24, 2022
BlackMario32
Yes you add a gram of activated charcoal.
Activated charcoal agar helps it grow faster and stronger from my experience... and its antibacterial..i use the same cups for media as well...
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February 24, 2022