I am very new to cloning and have questions....background: I have a jar of liquid culture that started to underperform after a year. My thought process was that I would fuck around with agar (how to make it, work with it) and the culture to see if I could grow out another tub and then clone the fatty. SO...The blue agar is 3 weeks old and inoculated with the LQ, A week later I made the green agar and placed tissue from a fruit. They are stored on a shelf. Both are parafilmed, What is that more aggressive looking web?! Is it some other kind of growth but still mycellial? The normal growth on the blue just showed up yesterday....like secondary to the weird spindly one. I'm just super curious about this
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Traditionalistic
The thick slimy tan stuff in both cups is bacteria. Not surprising, as LC tends to go bacterial. As others have said, repeatedly take very small samples of the healthiest tissue and transfer. Probably best to start with an antibiotic agar like this one here: https://fungi.com/products/antibiotic-ma... View More
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January 5, 2022
Traditionalistic
If you have fresh fruits, best to just transfer inner stem tissue directly to agar and not bother with the contaminated LC
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January 5, 2022
invitroHulk
I do open air too you just gotta know where in your work space is safe... I would try a different corner of my workspace because youre gonna always get some level of contam imo
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January 8, 2022
Luke_Skywalker
Shaboy some reading for your future project
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April 1, 2022